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重組人IFN alpha 2B
Recombinant Human IFN alpha 2B
產品編號: HZ-1070
規  格:
單  價: 詢價
生 產 商: Humanzyme
到貨時間: 3-4weeks
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Product Description:

 

  Human Cell Expressed
  Animal-derived product free
  Endotoxin-free*
  Available in Bulk
  Human Cell Expressed
  High Activity

HumanKine® IFNα 2B is a is expressed in human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 16 kDa. This cytokine is produced in a serum-free, chemically defined media. Production in human 293 cells offers authentic glycosylation which contributes to stability in cell growth media and other applications. The protein is highly active with a typical ED50 of 0.02-0.08 ng/mL. The cytokine is more than 95% pure.

Technical Specifications:

 

  Species: Human
  Expression: Human Cell Expressed
  Activity: 0.02-0.08 ng/mL ED50
  Purity: >95%
  Endotoxin: Endotoxin-free*
  Molecular Mass: 16 kDa monomer, glycosylated.

Activity Assay:

The specific activity was determined by the dose-dependent proliferation of WISH (heteroploid human amnion cell line) in presence of VSV ( vesicular stomatitis virus).

Purity Confirmation:

The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.


*Endotoxin-free: <0.5 EU/µg

All HumanKine proteins are expressed in the HumaXpress® human cell expression system and have low endotoxin levels with native human post-translational modifications and folding.

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Stem cell reserch
HumanZyme develops and manufactures growth factors and cytokines for stem cell research applications. HumanKine® cytokines and growth factors are applied to stem cells for culture expansion or to drive differentiation into specific cell types. The human cell manufacturing system ensures native human glycosylation and other human-specific modifications that can result in better bioactivity and enhanced half-life.

Immunology Research
HumanZyme develops and manufactures cytokines and cell culture media for immunology applications. Cytokines are cell signaling molecules that enable cell-cell communication, stimulation, differentiation, and other processes. HumanKine® cytokines, expressed in human cells, possess authentic human glycosylation, folding, disulfide bonds, and other modifications which are important for biological activity, half-life, and human-specific functions.

Human Cellular Therapy
HumanKine® cytokines and growth factors originally developed for stem cell applications are beginning to be utilized by cellular therapy developers in early stage trials. Growth factors and cytokines are often employed ex vivo to drive stem cell differentiation into potentially therapeutic cells. For many growth factors and cytokines, the industry lacks cGMP proteins needed for late stage clinical trials and subsequent regulatory approval of promising cellular therapies. HumanKine proteins can be adapted to cGMP production.
 

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