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重組人FGF basic-耐高溫
Recombinant Human FGF basic TS(Thermostable)
產品編號: HZ-1285
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單  價: 詢價
生 產 商: HumanZyme
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Product Description:

FGF Basic is a heparin-binding growth factor and is critical for human embryonic stem cells to remain in an undifferentiated state during cell culture. However, FGF basic’s half-life is so short that daily media changes are needed to maintain and expand stem cells. HumanKine® FGF-Basic TS™ is engineered for enhanced thermal-stability without modification of biological function, and was developed to improve stem cell culturing and expansion protocols.

FGF-Basic TS from HumanZyme is more resistant to degradation than native FGF-Basic.

FGF-Basic TS is more resistant to degradation than native FGF Basic when incubated at 37°C in cell culture media. In fact, HumanZyme’s collaborators’ experiments have demonstrated that cell culture media changes, when FGF basic TS replaced regular FGFbasic, can be performed every two or three days rather than daily. The collaborators also showed that the stem cell culture retains both pluripotency and good cell viability.

Technical Specifications:

  Species: Human
  Expression: Human Cell Expressed (HEK293)
  Activity: ≤ 0.5 ng/mL ED50
  Purity: >95%
  Endotoxin: Endotoxin-free*
  Molecular Mass: 17 kDa, monomer, 154 aa, non-glycosylated

Activity Assay:

The specific activity was determined by the dose-dependent stimulation of the proliferation of the 3T3 cell line.

Purity Confirmation:

The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.


*Endotoxin-free: <0.5 EU/µg

All HumanKine proteins are expressed in the HumaXpress® human cell expression system and have low endotoxin levels with native human post-translational modifications and folding.

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Stem cell reserch
HumanZyme develops and manufactures growth factors and cytokines for stem cell research applications. HumanKine® cytokines and growth factors are applied to stem cells for culture expansion or to drive differentiation into specific cell types. The human cell manufacturing system ensures native human glycosylation and other human-specific modifications that can result in better bioactivity and enhanced half-life.

Immunology Research
HumanZyme develops and manufactures cytokines and cell culture media for immunology applications. Cytokines are cell signaling molecules that enable cell-cell communication, stimulation, differentiation, and other processes. HumanKine® cytokines, expressed in human cells, possess authentic human glycosylation, folding, disulfide bonds, and other modifications which are important for biological activity, half-life, and human-specific functions.

Human Cellular Therapy
HumanKine® cytokines and growth factors originally developed for stem cell applications are beginning to be utilized by cellular therapy developers in early stage trials. Growth factors and cytokines are often employed ex vivo to drive stem cell differentiation into potentially therapeutic cells. For many growth factors and cytokines, the industry lacks cGMP proteins needed for late stage clinical trials and subsequent regulatory approval of promising cellular therapies. HumanKine proteins can be adapted to cGMP production.
 

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